Microscopes Microscope Accessories Categories. Quick Order. Bromothymol Blue pH Indicator, 1 oz. Concentration: 0. DOT: Not Regulated. Other Items In This Category. Add To Cart. Bromothymol Blue pH Indicator, 4 oz. Hydrion Plastic pH Strips, Bromothymol Blue pH Indicator, 16 oz.
Phenolphthalein pH Indicator, 1 oz. Phenolphthalein pH Indicator, 4 oz. Phenolphthalein pH Indicator, 16 oz. Also Available You May Also Like. Recently Viewed. Acetone, 20L ACS, Sample Header Text. Covalently-Bonded Unit. Yellow - in acidic solutions; green - in neutral solutions; blue - in basic solutions. Bromothymol blue ph. Bromothymol blue is synthesized by adding elemental bromine to the thymol blue in a solution of glacial acetic acid. To prepare a solution that is used as a pH indicator, we should dissolve 0.
To prepare a solution used as an indicator in volumetric work, we should dissolve 0. Let us discuss the major uses of bromothymol blue in detail.
Bromothymol blue can be used either to observe photosynthetic activities or as a respiratory indicator which turns yellow as CO 2 is added. A common demonstration of the pH indicator properties of BTB involves exhaling through a tube into the neutral solution of Bromothymol blue.
As the carbon dioxide is absorbed from the breath into the solution, the solution changes its color to yellow from green by forming carbonic acid. Therefore, BTB can be used commonly in science classes to demonstrate as, "the more that the muscles are used, the greater the carbon dioxide output. Bromothymol blue indicator has been used in conjunction with phenol red in monitoring the fungal asparaginase enzyme activity, with the phenol red turning pink.
And, the bromothymol blue turns blue by indicating an increase in pH and thus enzyme activity. However, one recent study suggests that the methyl red part is more useful in determining the activity because of the bright yellow ring form in the enzyme activity zone. It can also be used in the laboratory as a stain of a biological slide. It is already blue at this point, and a few drops are used on the water slide.
The cover-slip is also placed on the top of the water droplet, including the specimen in it, mixed with blue coloring. Sometimes, it is also used to define nuclei or cell walls under the microscope. Bromothymol can be used in obstetrics for detecting the premature rupture of membranes. Typically, the amniotic fluid has a pH value of greater than 7. Normally, as the vaginal pH is acidic, its blue color indicates the amniotic fluid's presence.
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